Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome

WU Ling-ling; LING Hua-yun; ZHOU Ying; QIU Li-juan; WANG Hong; XUE Yu; CHEN Hui-juan; WANG Ting-rui; WANG Bin

doi:10.16462/j.cnki.zhjbkz.2022.07.006

Volume 26 Issue 7

Jul. 2022

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2022 > 26(7): 777-783

WU Ling-ling, LING Hua-yun, ZHOU Ying, QIU Li-juan, WANG Hong, XUE Yu, CHEN Hui-juan, WANG Ting-rui, WANG Bin. Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006

Citation:

WU Ling-ling, LING Hua-yun, ZHOU Ying, QIU Li-juan, WANG Hong, XUE Yu, CHEN Hui-juan, WANG Ting-rui, WANG Bin. Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006

Citation:

WU Ling-ling, LING Hua-yun, ZHOU Ying, QIU Li-juan, WANG Hong, XUE Yu, CHEN Hui-juan, WANG Ting-rui, WANG Bin. Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006

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Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome

doi: 10.16462/j.cnki.zhjbkz.2022.07.006

Department of Epidemiology and Health Statistics, School of Public Health, Anhui Medical University, Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Hefei 230032, China

Funds:

National Natural Science Foundation of China 81573217

More Information

Corresponding author: WANG Bin, E-mail: wangbin@ahmu.edu.cn
Received Date: 2021-10-31
Rev Recd Date: 2022-02-15

Available Online: 2022-07-19

Publish Date: 2022-07-10

Abstract

Abstract

Objective The competing endogenous RNAs (ceRNA) regulation network in salivary gland tissues of patients with primary Sjögren's syndrome (pSS) was constructed by bioinformatics methods and its pathogenesis was investigated. Methods Differential expression analyses of messenger RNA (mRNA) dataset and long non-coding RNA (lncRNA) dataset from salivary gland tissues of pSS patients were conducted based on GEO database. Target miRNA and target mRNA related to pSS were obtained through miRNA databases, and then lncRNA-miRNA-mRNA ceRNA regulation network was constructed. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed for differentially expressed mRNAs (DEmRNAs) in the ceRNA regulation network. Results A total of 214 target mRNA were obtained in the ceRNA network. KEGG analysis of different genes in the network showed that the main gene enrichment pathway were mitogen-activated protein kinase (MAPK) signaling pathway and AMP-activated protein kinase (AMPK) signaling pathway. Hub gene JUN, CCND1, NRAS, DDX5, KAT2B, VEGFA, SIRT1, CLTA, MCM7 and RPS6KA1 were selected in pSS. Conclusion This study finds that the DEmRNAs in the ceRNA regulatory network mainly enriched in MAPK and AMPK signaling pathways, which provided a new idea for the diagnosis and treatment of primary Sjogren's syndrome.
- Primary Sjören's syndrome,
- CeRNA regulation network,
- Bioinformatics analysis,
- Messenger RNA,
- Long non-coding RNA,
- MicroRNA

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References(32)

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