Abstract: Objective To investigate dengue fever related knowledge, related attitude and behavior in university students in Guangzhou, and to provide scientific evidence for the university in order to efficiently control the spread of dengue fever and carry out prevention work. Methods A total of 1 564 students selected by random cluster sampling were surveyed with questionnaire. Results In the first two months before the investigation, 1.4 % students were diagnosed with dengue fever. About 48.5% students were afraid of being infected. The average score of knowledge was (72.7±16.4), different majors and areas also had statistical significance. The total protective behavior rate of the students was 69.3%. About 85.0% students could drain water and clean up dormitory timely. The dengue fever knowledge was obtained mainly through internet(67.6%). Multivariate Logistic regression analysis showed that the gender, major, grade, area, knowledge and attitude were the influencing factors of dengue prevention behavior. Conclusions The dengue infection rate of surveyed students was high. Except having positive attitude, knowledge and behavior of prevention are still need to strengthen and improve. Colleges should enhance education and relevant prevention measures to avoid the dengue fever be reignited.