Abstract: Objective To explore the risk factors of scrub typhus in Guangzhou City, and to provide the basis for making preventive and controlling measures of scrub typhus. Methods Case-control study was used with the ratio of 1 to 1. A total of 202 patients were selected as cases and 202 healthy residents matched for age and gender were recruited as controls. Face-to-face interview was conducted with unified design survey questionnaire and the exposure history of suspicious risk factors was collected. Data was analyzed by single factor described statistics and multivariable Logistic regression in SPSS 20.0. Results The results of single factor described statistics showed that there were significant differences in the history of outdoor activity or travel, the high frequency of outdoor activity, house tying and living in the house near grassland, vegetable field or ditch between case group and control group(all P<0.05). Multivariable logistic regression analysis showed that the history of outdoor activity or travel, the high frequency of outdoor activity and living in the house near grassland, vegetable field or ditch were risk factors (all P<0.05),with the odd ratio and 95% confidence interval of 10.124(95% CI:2.220-46.163), 2.348 (95% CI:1.431-3.853) and 1.801 (95% CI:1.180-2.748) respectively. Conclusions The history of outdoor activity or travel, the high frequency of outdoor activity and living in the house near grassland, vegetable field or ditch are risk factors of scrub typhus in Guangzhou City.