Abstract: Objective To assess the prevalence of musculoskeletal diseases in knee as well as the influencing factors among greenhouse farmers in Shouguang. Methods A cross-sectional household survey was conducted in the greenhouse farmers who had been working for at least a year. The greenhouse farmers were chosen from five main vegetable cultivating areas in Shouguang, Shandong Province. Results A total of 1 780 subjects were surveyed. The annual prevalence of knee musculoskeletal diseases among greenhouse farmers was 41.9%. 48 variables were found significantly associated with knee musculoskeletal diseases through univariate Logistic regression analysis. Multivariate Logistic regression analysis showed that the female gender (OR=1.795, 95% CI:1.433-2.249), age≥50years old (OR=1.635, 95% CI:1.271-2.105), BMI≥24 kg/m² (OR=1.445, 95% CI:1.151-1.814), working years≥20years (OR=1.767, 95% CI:1.368-2.282), keep working on knees(OR=1.616;95% CI:1.207-2.165), keep walking while working(OR=1.967;95% CI:1.290-3.000), lifting heavy objects in awkward posture (OR=1.421,95% CI:1.116-1.809), bending slightly(OR=2.084,95% CI:1.427-3.045), turning over significantly (OR=1.291,95% CI:1.002-1.663), bending or turning around the head repetitively (OR=1.565,95% CI:1.187-2.063) and forced bending(OR=1.245,95% CI: 0.979-1.584) were the main risk factors of musculoskeletal diseases in knees. Conclusions The findings showed a relatively higher prevalence of musculoskeletal diseases of knees among greenhouse farmers. Proper measures to prevent and control the prevalence of musculoskeletal diseases of knees are strongly recommended.