2013-2017年西安市食物中毒中金黄色葡萄球菌分型分析

栾阳; 金子懿; 张晔; 魏晓光; 王增国

doi:10.16462/j.cnki.zhjbkz.2019.02.018

2013-2017年西安市食物中毒中金黄色葡萄球菌分型分析

doi: 10.16462/j.cnki.zhjbkz.2019.02.018

1.
710054 西安, 西安市疾病预防控制中心微生物检验科
2.
710068 西安, 陕西国际旅行卫生保健中心检验科

详细信息

通讯作者:
王增国, E-mail: wangzengguo@xiancdc.cn

中图分类号: R181
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出版历程
- 收稿日期: 2018-10-09
- 修回日期: 2018-11-28
- 刊出日期: 2019-02-10

Molecular Epidemiological Characteristics of Staphylococcus aureus Strains Associated with Food Poisoning in Xi'an from 2013 to 2017

1.
Microbiology Laboratory, Xi'an Municipal Center for Disease Control and Prevention, Xi'an 710054, China
2.
Clinical Laboratory, Shaanxi International Travel Healthcare Center, Xi'an, Shaanxi 710068, China

More Information

Corresponding author: WANG Zeng-guo, E-mail: wangzengguo@xiancdc.cn

摘要

摘要: 目的了解2013-2017年西安市食物中毒中分离的金黄色葡萄球菌（staphylococcus aureus，S.aureus）分子流行特点。方法通过脉冲场凝胶电泳（pulsed field gel electrophoresis，PFGE）、多位点序列分型（multilocus sequence typing，MLST）和spa分型方法对八起食物中毒标本的26株金黄色葡萄球菌进行分子分型，运用聚合酶链式反应（polymerase chain reaction，PCR）检测相关肠毒素基因。结果 26株S.aureus PFGE可分为7种型别（A-G）；MLST可分为3种序列型（ST6，ST5和ST59）。其中，ST6占65.38%，为主要型别；spa可分为4种型别（t437，t701，t11363和t5923），优势型别为t701，占65.38%。肠毒素基因检测中，sea基因占76.92%（20/26）；sed-seg基因占23.08%（6/26）；其它肠毒素基因未检出。结论 2013-2017年西安市食物中毒标本中分离培养的S.aureus的主要流行型别为ST6-t701-sea，并首次检出含有seg新型肠毒素基因。PFGE、MLST和spa分型三种方法相结合全面的了解菌株的分子流行特点。
- 金黄色葡萄球菌 /
- 分子分型 /
- 食物中毒
Abstract: Objective To understand the molecular epidemiological characteristics of staphylococcus aureus(S.aureus), which was isolated from food poisoning in Xian between 2013 and 2017. Methods A total of 26 S.aureusisolates from 8 outbreaks were analyzed by using pulsed field gel electrophoresis(PFGE), multilocus sequence typing(MLST) and spa typing. polymerase chain reaction(PCR) analysis was used to analyze the staphylococcal enterotoxin genes sea to sei. Results 26 strains of S.aureus were divided into 7 types of A-G by PFGE; They were divided into 3 types by MSLT, ST6 accounted for 65.38% which was the main type; They were Divided into 4 types by spa typing, t701 accounted for 65.38% which is the dominant type. The sea accounted for 76.92% (20/26) in the enterotoxin gene testing; sed-seg accounted for 23.08% (6/26); other enterotoxin genes were not detected. Conclusion The predominant type of S.aureus isolated from food poisoning in Xi'an was ST6-t701-seaprofile from 2013 to 2017. The first detection of the new enterotoxin gene seg, PFGE, MLST and spa typing were combined to fully understand the molecular epidemic characteristics of strains.
- Staphylococcus aureus /
- Molecular typing /
- Food poisoning

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图 1 8起食物中毒PFGE，MLST和spa聚类分析图

Figure 1. Cluster analysis of PFGE, MLST and spa in 8 cases of food poisoning

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表 1 8起S.aureus食物中毒流行病学数据

Table 1. Epidemiological data for 8 cases of S. aureus food poisoning

	日期	地点	人数	潜伏期(h)	症状	中毒食物
1	2013/06/13	小吃城	4	2.0~4.0	呕吐	炒饭
2	2014/05/06	学校	10	1.5~5.0	呕吐、腹泻	土耳其烤肉
3	2015/01/12	幼儿园	5	1.5~4.0	呕吐、恶心	三明治、饼干
4	2016/05/04	学校	8	2.0~5.0	呕吐、腹泻	蒜苔炒肉、包菜、炒青椒
5	2016/060/6	小吃城	4	1.0~3.0	呕吐、腹泻	烤鸡肉
6	2016/08/01	酒店	8	2.0~6.0	呕吐、腹泻	排骨、炒饭、肥牛、蛋糕、色拉卷、果仁脆
7	2016/10/16	酒店	5	1.5~3.0	呕吐、头疼	牛肉、马面鱼
8	2017/05/08	酒店	6	1.5~5.0	呕吐、腹泻	凉拌牛肉和黄瓜

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表 2 26株S.aureus分离来源及三种方法分型结果

Table 2. Sources of 26 S. aureus isolates and their typing results by three methods

序号	来源(菌株)	MLST	spa分型	PFGE	肠毒素基因
1	食物(1)	ST6	t701	C	sea
	肛拭子(1)	ST6	t701	C	sea
2	食物(1)	ST6	t701	D	sea
	呕吐物(1)	ST6	t701
	粪便(1)	ST6	t701
3	食物(2)	ST6	t701	B	sea
4	食物(3)	ST59	t11363	G	sea
5	粪便(2)	ST6	t701	A	sea
	呕吐物(1)	ST6	t701
	食物(1)	ST6	t701
6	食物(6)	ST6	t437	A	sea
7	食物(2)	ST5	t11363	E	sed-seg
8	食物(3)	ST5	t5923	F	sed-seg
	环境(1)	ST5	t5923	F	sed-seg

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