TNF-α基因rs1800629多态性与中国人群宫颈癌的易感性

丁波; 孙玮; 沈杨; 蔡云朗; 王适之

doi:10.16462/j.cnki.zhjbkz.2019.07.021

TNF-α基因rs1800629多态性与中国人群宫颈癌的易感性

doi: 10.16462/j.cnki.zhjbkz.2019.07.021

丁波¹,
孙玮²,
沈杨¹,
蔡云朗¹,
王适之^3, ,

1.
210009 南京, 东南大学附属中大医院妇产科
2.
210036 南京, 江苏省妇幼保健院妇产科
3.
210009 南京, 东南大学公共卫生学院

基金项目:

国家自然科学基金 81872684

江苏省自然科学基金 BK20171367

详细信息

通讯作者:
王适之, E-mail: shizhiwang2009@seu.edu.cn

中图分类号: R711.74
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- 被引次数: 0
出版历程
- 收稿日期: 2019-01-22
- 修回日期: 2019-04-01
- 刊出日期: 2019-07-10

TNF-α rs1800629 polymorphisms in the genetic susceptibility to cervical cancer

1.
Department of Obstetrics and Gynecoloty, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China
2.
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China
3.
School of public health, Southeast university, Nanjing 210009, China

Funds:

National Natural Science Foundation of China 81872684

Natural Science Foundation of Jiangsu Province BK20171367

More Information

Corresponding author: WANG Shi-zhi, E-mail: shizhiwang2009@seu.edu.cn

摘要

摘要: 目的探讨肿瘤坏死因子-α(tumor necrosis factor α，TNF-α)基因位点rs1800629单核苷酸多态性与宫颈癌发生的关系。方法选择经组织学确诊的汉族552例新发宫颈癌患者作为病例组，与病例组人群不存在生物学相关的654例正常人群作为对照组，利用Taqman实时荧光定量聚合酶链式反应(polymerase chain reaction，PCR)技术检测基因型，Logistic回归模型计算基因型与人群罹患宫颈癌的风险比(odds ratio，OR)及其95%的可信区间(95%CI)；Stata 11.0软件对中国人群rs1800629多态性与宫颈癌的关系进行Meta分析。结果与TNF-α rs1800629野生型GG基因型的相比，个体携带rs1800629 GA、AA及GA/AA基因型的个体罹患宫颈癌的风险差异无统计学意义(均有P>0.05)；对该位点多态性与宫颈癌关系的Meta分析发现，TNF-α rs1800629遗传变异与中国人群宫颈癌的发生无明显相关性。结论 TNF-α基因rs1800629位点多态性可能与中国人群宫颈癌发生无关。
- 肿瘤坏死因子-α /
- rs1800629 /
- 多态性 /
- 宫颈癌
Abstract: Objective To investigate the relationship between tumor necrosis factor α (TNF-α) rs1800629 polymorphisms and cervical cancer risk. Methods A case-control study was carried out including 552 patients with cervical cancer and 654 normal controls during the same period. TNF-α rs1800629 polymorphisms were examined by Taqman-Probe assay method. The association between the genotypes and cervical cancer was analyzed by Logistic regression models. Stata 11.0 was used for the Meta-analysis. Results Compared with the TNF-α rs1800629 GG genotype, individuals with GA, AA and GA/AA genotypes showed no significant changes in the risk of cervical cancer (all P>0.05). Further Meta-analysis on the relationship between the polymorphisms of TNF-α rs1800629 and cervical cancer also suggested that there was no significant correlation between the genetic variation and the occurrence of cervical cancer. Conclusion The polymorphisms of TNF-α rs1800629 may not be related to cervical cancer risk in Chinese population.
- TNF-α /
- rs1800629 /
- Polymorphism /
- Cervical cancer

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图 1 文献筛选的流程图

Figure 1. Flow chart of literature screening

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图 2 TNF-α rs1800629多态性(GA/AA VS GG)与宫颈癌风险的森林图

Figure 2. Forest plot of cervical cancer risk associated with TNF-α rs1800629 (GA/AA VS GG)

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图 3 TNF-α rs1800629多态性与宫颈癌风险(GA/AA VS GG)的Galbraith图

Figure 3. Galbraith plot assessing sources of heterogeneity from 8 studies

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图 4 TNF-α rs1800629多态性与宫颈癌风险(GA/AA VS GG)的漏斗图

Figure 4. Funnel plot assessing evidence of publication bias from 8 studies (GA/AA VS GG)

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表 1 宫颈癌病例和对照的统计学变量

Table 1. Demographic and selected variables in cervical cancer cases and controls

变量	病例组(n=552)	对照组(n=654)	P值
年龄[岁, (x±s)]	47.46±10.10	47.34±10.60	0.834
初次生育年龄[岁, (x±s)]	23.82±2.37	25.12±2.67	＜0.001
产次[次，n(%)]			＜0.001
0~1	328(59.4)	501(76.6)
≥2	224(40.6)	153(23.4)
绝经[n(%)]			0.407
是	200(36.2)	222(33.9)
否	352(63.8)	432(66.1)
临床分期[n(%)]
Ⅰ	379
Ⅱ	139
Ⅲ	26
Ⅳ	7

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表 2 TNF-α rs1800629基因多态性与宫颈癌易感性的关系[n(%)]

Table 2. TNF-α rs1800629 polymorphisms and cervical cancer [n(%)]

基因型	宫颈癌组(n=552)	对照组(n=654)	宫颈癌组vs对照组
基因型	宫颈癌组(n=552)	对照组(n=654)	P值	调整OR(95% CI)^a值
rs1800629
GG	481(87.1)	565(86.4)		1.000
GA	66(12.0)	84(12.8)	0.648	0.923(0.654~1.303)
AA	5(0.9)	5(0.8)	0.800	1.175(0.338~4.082)
GA/AA	71(12.9)	89(13.6)	0.703	0.937(0.670~1.310)
G等位	1 028(93.1)	1 214(92.8)		1.000
A等位	76(6.9)	94(6.2)	0.772	0.955(0.698~1.306)
注：^a调整了年龄、产次和是否绝经因素。

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表 3 纳入TNF-α rs1800629多态性的Meta分析研究的基本信息

Table 3. Main characterics of TNF-α rs1800629 studies included in the Meta-analysis

作者	年份	地区	分型方法	病例/对照	病例组			对照组			P-HWE(对照组)
作者	年份	地区	分型方法	病例/对照	AA	AG	GG	AA	AG	GG	P-HWE(对照组)
祖菲娅	2010	新疆	PCR-RFLP	111/100	50	54	7	72	18	10	＜0.001
Wang	2011	西安	PCR-RFLP	186/200	149	30	7	144	46	10	0.019
Huang	2012	蚌埠	PCR-RFLP	42/87	33	6	3	64	20	3	0.372
Wang	2012	辽宁	PCR-RFLP	285/274	247	30	8	274	35	9	＜0.001
Li	2018	山东	TaqMan	452/494	317	135	0	424	70	0	0.090
Li	2018	齐齐哈尔	PCR-RFLP	142/150	114	24	4	125	22	3	0.102
Du	2018	四川	Sanger测序	522/550	390	84	48	431	91	28	＜0.001
Ding	2018	江苏	TaqMan	552/654	481	66	5	565	84	5	0.342
注：PCR：聚合酶链式反应；RFLP：限制性片段长度多态性。

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表 4 TNF-α rs1800629多态性与宫颈癌风险的Meta分析结果

Table 4. Results of Meta-analysis for TNF-α rs1800629 polymorphism and cervical cancer risk

研究	数目	病例/对照	GG vs AA		AG vs AA		显性模型		隐形模型		G等位vs A等位
研究	数目	病例/对照	OR (95% CI)值	P值^a	OR (95% CI)值	P值^a	OR (95% CI)值	P值^a	OR (95% CI)值	P值^a	OR (95% CI)值	P值^a
Total	8	1 770/2 003	1.02 (0.64~1.63)	0.912	1.26 (0.75~2.13)	＜0.001	1.25 (0.78~2.01)	＜0.001	0.94 (0.59~1.50)	0.798	1.19 (0.83~1.72)	＜0.001
HWE平衡	4	1 188/1 385	1.42 (0.62~3.26)	0.893	1.22 (0.63~2.37)	＜0.001	1.28 (0.69~2.38)	＜0.001	1.45 (0.63~3.33)	0.851	1.30 (0.78~2.18)	0.001
注：^a运用Q检验对各组研究间的异质性进行检验，如异质性P≥0.1，选择固定效应模型，否则选择随机效应模型。

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