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河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究

刘万双 陈浩荣 代科 蒋宝贵 陈津津 田珅 吴家红 刘玮 方立群

刘万双, 陈浩荣, 代科, 蒋宝贵, 陈津津, 田珅, 吴家红, 刘玮, 方立群. 河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究[J]. 中华疾病控制杂志, 2024, 28(1): 83-91. doi: 10.16462/j.cnki.zhjbkz.2024.01.013
引用本文: 刘万双, 陈浩荣, 代科, 蒋宝贵, 陈津津, 田珅, 吴家红, 刘玮, 方立群. 河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究[J]. 中华疾病控制杂志, 2024, 28(1): 83-91. doi: 10.16462/j.cnki.zhjbkz.2024.01.013
LIU Wanshuang, CHEN Haorong, DAI Ke, JIANG Baogui, CHEN Jinjin, TIAN Shen, WU Jiahong, LIU Wei, FANG Liqun. Investigation on infection of severe fever with thrombocytopenia syndrome virus in two animals in Shangcheng County, Henan Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(1): 83-91. doi: 10.16462/j.cnki.zhjbkz.2024.01.013
Citation: LIU Wanshuang, CHEN Haorong, DAI Ke, JIANG Baogui, CHEN Jinjin, TIAN Shen, WU Jiahong, LIU Wei, FANG Liqun. Investigation on infection of severe fever with thrombocytopenia syndrome virus in two animals in Shangcheng County, Henan Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(1): 83-91. doi: 10.16462/j.cnki.zhjbkz.2024.01.013

河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究

doi: 10.16462/j.cnki.zhjbkz.2024.01.013
基金项目: 

国家重点研发计划项目 2019YFC1200604

详细信息
    通讯作者:

    方立群,E-mail: fang_lq@163.com

  • 中图分类号: R183.5

Investigation on infection of severe fever with thrombocytopenia syndrome virus in two animals in Shangcheng County, Henan Province

Funds: 

The Key Research and Development Program of China 2019YFC1200604

More Information
  • 摘要:   目的  通过开展发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome, SFTS)自然疫源地动物宿主感染新布尼亚病毒(severe fever with thrombocytopenia syndrome virus, SFTSV)的调查研究,明确SFTS自然疫源地SFTSV的传播风险与影响因素,为SFTS的针对性防控提供科学依据。  方法  采用卫星定位技术连续跟踪商城县邱湾村、平塘村和龙头桥村3个村庄部分村民家养狗日常活动轨迹2周并采集血清样本,使用间接荧光抗体试验(indirect immunofluorescence assay,IFA)法检测家养狗血清SFTSV-IgG抗体,使用logistic回归分析模型分析影响家养狗感染SFTSV的潜在环境影响因素;采用夹(笼)夜法和粘鼠板法对家养狗活动区域内的啮齿动物及小型哺乳动物种群进行密度调查,针对保守基因进行PCR种类鉴定,并采用荧光PCR和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测啮齿动物及小型哺乳动物SFTSV感染情况。  结果  通过卫星定位共追踪26条家养狗的日常活动范围信息,采集37条家养狗52份血清,SFTSV-IgG抗体总阳性率为59.46%;共捕获啮齿动物及小型哺乳动物419只,优势种为黄胸鼠、小家鼠、黑线姬鼠,未有SFTSV阳性标本检出;家养狗活动中心离主干道路距离远和到过林地的频次多是感染SFTSV的危险因素,家养狗活动中心离林地距离远是感染SFTSV的保护因素。  结论  商城县家养狗SFTSV抗体阳性率较高,感染SFTSV与生态环境相关,建议进一步扩大家养动物的种类,开展SFTSV感染情况的研究;啮齿动物的SFTSV感染率低,在SFTSV自然循环过程中的作用需进一步调查。
  • 图  1  检查家养狗身上蜱虫附着情况、佩戴定位仪及采集血清

    Figure  1.  Check for tick attachment, wear a locator, and collect serum

    图  2  不同村庄鼠密度和鼠种在不同生境分布图

    Figure  2.  Distribution of rodent density and species in different habitats in different villages

    表  1  扩增引物序列

    Table  1.   Amplification primer sequences

    扩增片段
    Amplified fragments
    引物名称
    Primer name
    扩增引物序列(5′-3′)
    Amplified primer sequence(5′-3′)
    退火温度/℃
    Annealing temperature/℃
    CytB L7 ACCAATGACATGAAAAATCATCGTT 55
    H15915 TCTCCATTTCTGGTTTACAAGAC
    SFTSV BNY-F AGCCTAATTGGATATGTCAAATTGC 60
    BNY-R CGGGTGAAGTGGCTGAAGG
    BNY-P FAM-AGCAGCAGCAGCAACCTCAGCAGC-BHQ1
    注:SFTS,发热伴血小板减少综合征。
    Note: SFTS, severe fever with thrombocytopenia syndrome.
    下载: 导出CSV

    表  2  纳入logistic回归模型分析的变量

    Table  2.   Variables included in logistic regression model

    变量Variable 变量描述Variable description 类型Type
    耕地占比
    Proportion of cultivated land
    缓冲区内耕地面积所占百分比/%
    Percentage of cultivated land in the buffer zone /%
    连续变量
    Continuous
    林地占比
    Proportion of forest land
    缓冲区内林地面积所占百分比/%
    Percentage of forest area in the buffer zone /%
    连续变量
    Continuous
    水域占比
    Proportion of water body
    缓冲区内水域面积所占百分比/%
    Percentage of water body in the buffer zone /%
    连续变量
    Continuous
    建设用地占比
    Proportion of construction land
    缓冲区内建设用地面积所占百分比/%
    Percentage of construction land area in the buffer zone /%
    连续变量
    Continuous
    其他用地占比
    Proportion of other land use
    缓冲区内其他用地面积所占百分比/%
    Percentage of other land area in the buffer zone /%
    连续变量
    Continuous
    林地距离
    Forest land distance
    家养狗活动中心点到最近林地的距离/m
    Distance from domestic dog activity center to nearest forest land /m
    连续变量
    Continuous
    耕地距离
    Cultivated land distance
    家养狗活动中心点到最近耕地的距离/m
    Distance from domestic dog activity center to nearest cultivated land /m
    连续变量
    Continuous
    水域距离
    Water body distance
    家养狗活动中心点到最近水域的距离/m
    Distance from domestic dog activity center to nearest water body /m
    连续变量
    Continuous
    主干道路距离
    Trunk road distance
    家养狗活动中心点到最近主干道路的距离/m
    Distance from domestic dog activity center to nearest trunk road /m
    连续变量
    Continuous
    耕地频次
    Cultivated land frequency
    狗到耕地的频次
    Frequency of dog visits to cultivated land
    连续变量
    Continuous
    林地频次
    Forest land frequency
    狗到林地的频次
    Frequency of dog visits to forest land
    连续变量
    Continuous
    水域频次
    Water body frequency
    狗到水域的频次
    Frequency of dog visits to water body
    连续变量
    Continuous
    建设用地频次
    Construction land frequency
    狗到建设用地的频次
    Frequency of dog visits to construction land
    连续变量
    Continuous
    鼠密度
    Rat density
    家养狗活动区域内鼠的密度/%
    Density of rats in the living area of domestic dogs /%
    连续变量
    Continuous
    鼠丰度
    Rat abundance
    家养狗活动区域内鼠的种类/种
    Species of rats in areas where domestic dogs live /Species
    连续变量
    Continuous
    下载: 导出CSV

    表  3  家养狗及啮齿动物SFTSV检测情况

    Table  3.   Detection of SFTSV in domestic dogs and rodents

    地点Location 家养狗Domestic dogs 啮齿动物Rodent
    IFA 阳性数(阳性率/%)
    Positive number (positive rate/%)
    RT-PCR 阳性数(阳性率/%)
    Positive number (positive rate/%)
    ELISA 阳性数(阳性率/%)
    Positive number (positive rate/%)
    邱湾村Qiuwan Village 8 7(87.50) 125 0(0.00) 73 0(0.00)
    平塘村Pingtang Village 6 4(66.67) 148 0(0.00) 96 0(0.00)
    龙头桥村Longtouqiao Village 23 11(47.83) 146 0(0.00) 81 0(0.00)
    合计Total 37 22(59.46) 419 0(0.00) 250 0(0.00)
    注: SFTS, 发热伴血小板减少综合征; IFA, 间接荧光抗体试验; RT-PCR, 反转录聚合酶链反应; ELISA, 酶联免疫吸附试验。
    Note: SFTS, severe fever with thrombocytopenia syndrome; IFA, indirect immunofluorescence assay; RT-PCR, reverse transcription-polymerase chain reaction; ELISA, enzyme linked immunosorbent assay.
    下载: 导出CSV

    表  4  商城县啮齿动物及小型哺乳动物采集情况

    Table  4.   Collection of rodents and small mammals in Shangcheng

    鼠种Rat species 邱湾村Qiuwan Village 平塘村Pingtang Village 龙头桥村Longtouqiao Village 总计
    Total
    林地
    Forest land
    花生地
    Peanut land
    杂草地
    Weedy land
    室内
    Interior room
    合计
    Total
    林地
    Forest land
    花生地
    Peanut land
    杂草地
    Weedy land
    室内
    Interior room
    合计
    Total
    林地
    Forest land
    花生地
    Peanut land
    杂草地
    Weedy land
    室内
    Interior room
    合计
    Total
    黄胸鼠Ruttus flauipectus 28 6 28 23 85 11 18 29 35 93 16 12 31 16 75 253
    小家鼠Mus musculus 5 2 4 4 15 3 2 5 2 12 11 5 19 20 55 82
    黑线姬鼠Apodemus agrarius 0 9 0 0 9 3 16 5 0 24 0 4 0 0 4 37
    北社鼠Chinese white-bellied rat 3 3 7 0 13 5 0 2 0 7 0 0 0 0 0 20
    褐家鼠Rattus norvegicus 0 0 1 0 1 1 1 4 0 6 2 0 8 0 10 17
    小鼩鼱Shrew 0 0 1 0 1 0 0 2 0 2 0 0 1 0 1 4
    赤腹松鼠Callosciurus erythraeus 1 0 0 0 1 2 0 0 0 2 1 0 0 0 1 4
    安氏白腹鼠Niviventer andersoni 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 2
    合计Total 37 20 41 27 125 25 37 49 37 148 30 21 59 36 146 419
    下载: 导出CSV

    表  5  家养狗感染SFTSV环境危险因素logistic回归分析

    Table  5.   Logistic regression analysis of environmental risk factors for SFTSV infection in dogs

    变量Variable 单因素分析Univariate analysis 多因素分析Multivariate analysis
    OR值value (95% CI) P值value OR值value (95% CI) P值value
    面积百分比/% Area percent/%
      耕地Cultivated land 0.89(0.80~0.97) 0.018 0.84(0.79~0.92) 0.073
      林地Forest land 1.08(1.00~1.19) 0.070 1.12(0.93~1.24) 0.068
      水域Water body 0.96(0.74~1.24) 0.715 0.77(0.62~0.98) 0.214
      建设用地Construction land 1.00(0.83~1.20) 0.973 0.99(0.81~1.07) 0.457
      其他用地Other land use 1.30(0.98~2.07) 0.129 1.24(0.95~1.96) 0.132
    距离/10 m Distance/10 m
      耕地Cultivated land 0.81(0.57~1.09) 0.183 0.83(0.80~1.15) 0.150
      林地Forest land 0.80(0.68~0.90) 0.002 0.76(0.58~0.89) 0.007
      水域Water body 1.27(0.95~1.80) 0.136 0.85(0.72~0.97) 0.127
      主干道路Trunk road 1.07(1.01~1.16) 0.033 1.13(1.03~1.29) 0.027
    频次 Frequency
      耕地Cultivated land 1.00(1.00~1.01) 0.070 1.00(0.93~1.05) 0.070
      林地Forest land 1.01(1.00~1.02) 0.005 1.01(1.00~1.02) 0.005
      水域Water body 1.01(0.99~1.03) 0.264 1.01(0.97~1.03) 0.208
      建设用地Construction land 1.00(1.00~1.00) 0.720 1.00(1.00~1.00) 0.350
      鼠密度Rat density 1.24(1.06~1.35) 0.170 1.16(1.07~1.37) 0.131
      鼠丰度Rat abundance 0.97(0.84~1.07) 0.240 0.99(0.82~1.91) 0.180
    注:① 1 000 m缓冲区内土地利用类型的面积百分比;②家养狗的活动中心点到最近的环境变量的距离;③家养狗到各种生境的次数。
    Note: ① Area percentage of land use types within the 1 000 m buffer zone; ② The distance from the domestic dog′s center of activity to the nearest environmental variable; ③ Number of visits by domestic dogs to various habitats.
    下载: 导出CSV
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    Ge HM, Wang QK, Li ZF, et al. Survey of SFTSV carrying situation in rodents in SFTS epidemic area of Donghai County[J]. Jiangsu J Prev Med, 2012, 23(6): 12-14. DOI: 10.3969/j.issn.1006-9070.2012.06.005.
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    [23] 范龙兴, 宁保安, 刘颖. 3S技术在疾病监控中的应用研究进展[J]. 解放军预防医学杂志, 2017, 35(3): 276-279. DOI: 10.13704/j.cnki.jyyx.2017.03.023.

    Fan LX, Ning BA, Liu Y. Research progress on the application of 3S technology in disease surveillance[J]. Prev Med Chin PLA, 2017, 35(3): 276-279. DOI: 10.13704/j.cnki.jyyx.2017.03.023.
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出版历程
  • 收稿日期:  2022-04-20
  • 修回日期:  2022-07-13
  • 网络出版日期:  2024-02-05
  • 刊出日期:  2024-01-10

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