Detection of ccc DNA and HBV DNA in peripheral blood mononuclear cells of patients with HBV infection by in situ hybridization

LIU Chang; ZHONG Yan-wei; XU Yuan-yong; DI Feng-lin; WU Yi-chen; LIU Hong-bo; LI Hao; JIANG Qi-yu; WANG Zhi-jie

doi:10.16462/j.cnki.zhjbkz.2016.09.007

Volume 20 Issue 9

Sep. 2016

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2016 > 20(9): 888-892

LIU Chang, ZHONG Yan-wei, XU Yuan-yong, DI Feng-lin, WU Yi-chen, LIU Hong-bo, LI Hao, JIANG Qi-yu, WANG Zhi-jie. Detection of ccc DNA and HBV DNA in peripheral blood mononuclear cells of patients with HBV infection by in situ hybridization[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(9): 888-892. doi: 10.16462/j.cnki.zhjbkz.2016.09.007

Citation:

LIU Chang, ZHONG Yan-wei, XU Yuan-yong, DI Feng-lin, WU Yi-chen, LIU Hong-bo, LI Hao, JIANG Qi-yu, WANG Zhi-jie. Detection of ccc DNA and HBV DNA in peripheral blood mononuclear cells of patients with HBV infection by in situ hybridization[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(9): 888-892. doi: 10.16462/j.cnki.zhjbkz.2016.09.007

Citation:

LIU Chang, ZHONG Yan-wei, XU Yuan-yong, DI Feng-lin, WU Yi-chen, LIU Hong-bo, LI Hao, JIANG Qi-yu, WANG Zhi-jie. Detection of ccc DNA and HBV DNA in peripheral blood mononuclear cells of patients with HBV infection by in situ hybridization[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(9): 888-892. doi: 10.16462/j.cnki.zhjbkz.2016.09.007

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Detection of ccc DNA and HBV DNA in peripheral blood mononuclear cells of patients with HBV infection by in situ hybridization

doi: 10.16462/j.cnki.zhjbkz.2016.09.007

1. Disease Surveillance Center of the PLA, Institute of Disease Control and Prevention, Beijing 100071, China;
2. Research Center for Clinical, 302 Hospital, Beijing 100039, China;
3. Department of Parasitesm, School of Laboratory, Hebei North University, Zhangjiakou 075000, China

Received Date: 2016-01-13
Rev Recd Date: 2016-05-09

Abstract

Abstract

Objective To investigate whether hepatitis B virus (HBV) can infect and copy in the peripheral blood mononuclear cell (PBMC) in patients with chronic hepatitis B. Methods 137 inpatient attenders' peripheral blood was selected;mononuclear cells were isolated from peripheral blood;cells were fixed using cell rejection slice method; HBV DNA and cccDNA were detected with Real-time Quantitative polymerouse chain rection(QPCR) and rolling circle amplification combined with in situ cross gap PCR immunohistochemical staining method was used to detect HBV DNA and HBV cccDNA expression in PBMC. Results In 137 cases of specimen, after immunohistochemical staining, 68.6% (94/137) cases were detected the expression of HBV DNA, 83.9% (115/137) cases were deteced of HBV cccDNA positive signal by in situ hybridization and rolling circle amplification PCR; the positive rate of HBV cccDNA in PBMC of patients with serum HBV DNA concentration with higher than 106 copies/ml was 95.1%, the positive rate of less than 106 copies/ml was 51.4%. Conclusions HBV can infect the peripheral blood mononuclear cells and replicate in it, this study lays the foundation for further study of the PBMC infection leading to intrauterine infection of HBV.
- Hepatitis B virus,
- DNA,
- Primed in situ labeling

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References(21)

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