Epidemiological and genetic characteristics of norovirus in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019

LI Jing; ZHANG Ting; ZOU Wen-jing; XU Jun-qiang; CAI Kun

doi:10.16462/j.cnki.zhjbkz.2021.11.017

Volume 25 Issue 11

Nov. 2021

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LI Jing, ZHANG Ting, ZOU Wen-jing, XU Jun-qiang, CAI Kun. Epidemiological and genetic characteristics of norovirus in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(11): 1332-1335. doi: 10.16462/j.cnki.zhjbkz.2021.11.017

Citation:

LI Jing, ZHANG Ting, ZOU Wen-jing, XU Jun-qiang, CAI Kun. Epidemiological and genetic characteristics of norovirus in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(11): 1332-1335. doi: 10.16462/j.cnki.zhjbkz.2021.11.017

Citation:

LI Jing, ZHANG Ting, ZOU Wen-jing, XU Jun-qiang, CAI Kun. Epidemiological and genetic characteristics of norovirus in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(11): 1332-1335. doi: 10.16462/j.cnki.zhjbkz.2021.11.017

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Epidemiological and genetic characteristics of norovirus in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019

doi: 10.16462/j.cnki.zhjbkz.2021.11.017

Institute of Health Examination, Hubei Province Center for Disease Control and Prevention, Wuhan 430079, China

Funds:

National Key Technology Research and Development Program of China 2017ZX10104001

Natural Science Foundation of Hubei Province 2018CFB716

More Information

Corresponding author: CAI Kun, E-mail: 56594776@qq.com
Received Date: 2021-01-18
Rev Recd Date: 2021-03-16

Available Online: 2021-12-04

Publish Date: 2021-11-10

Abstract

Abstract

Objective To describe the epidemiologic and genetic characteristics of norovirus (NV) in sentinel surveillance cases of viral diarrhea in Hubei Province from 2017 to 2019. Methods A total of 1 957 fecal samples from adult and child patients with diarrhea were collected from January 1, 2017 to December 30, 2019. Samples were detected using real-time RT-PCR. Positive samples of NV GII were amplified to obtain the sequences of open reading frame (ORF)1/ORF2 junction by RT-PCR. The obtained PCR products were directly sequenced and analyzed for genotype and recombination. Results There was a statistically significant difference in the detection rate of NV between males and females with diarrhea (χ² =5.18, P=0.023). There is a statistically significant difference in the detection rate of NV between patients with diarrhea in each age group (χ² =98.04, P < 0.001), among them, the 1~ < 2 year old age group has the highest NV-positive detection rate (23.50%) in patients with diarrhea. From 2017 to 2019, there were two epidemic peaks of NV infection in Hubei Province, the peak appeared in October-December, and the low peak appeared in March-April. The main NV genotypes were GII genogroup, of which GII.4, GII.2 and GII.3 were the main subtypes. The main recombination types were GII.2[P16], GII.4[P31], GII.3[P12] and GII.4[P16]. Conclusions There were multiple genotypes of NV infection in Hubei province from 2017 to 2019 and different recombination subtypes were found. Enhancing the monitoring of genotype and recombination of NV contributes to know the molecular epidemiology features of NV and provide theoretical basis for scientific prevention and control.
- Norovirus,
- Epidemiology,
- Genotype,
- Gene recombination

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References(24)

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