Genetic characteristics of coxsackie A4 virus in a cluster of human infections in Suzhou

DONG Ze-feng; XU Zhi-hui; WANG Di; WU Xue-fei; YA Xue-rong; XIA Yu; SHEN Qiang

doi:10.16462/j.cnki.zhjbkz.2023.03.021

Volume 27 Issue 3

Mar. 2023

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DONG Ze-feng, XU Zhi-hui, WANG Di, WU Xue-fei, YA Xue-rong, XIA Yu, SHEN Qiang. Genetic characteristics of coxsackie A4 virus in a cluster of human infections in Suzhou[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(3): 363-367. doi: 10.16462/j.cnki.zhjbkz.2023.03.021

Citation:

DONG Ze-feng, XU Zhi-hui, WANG Di, WU Xue-fei, YA Xue-rong, XIA Yu, SHEN Qiang. Genetic characteristics of coxsackie A4 virus in a cluster of human infections in Suzhou[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(3): 363-367. doi: 10.16462/j.cnki.zhjbkz.2023.03.021

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Genetic characteristics of coxsackie A4 virus in a cluster of human infections in Suzhou

doi: 10.16462/j.cnki.zhjbkz.2023.03.021

Laboratory of Pathogenic Microbiology, Suzhou Center for Disease Control and Prevention, Suzhou 215004, China

Funds:

Suzhou Key Technologies for the Prevention and Control of Major and Infectious Diseases GWZX202202

Suzhou Key Technologies for the Prevention and Control of Major and Infectious Diseases GWZX202002

The Key Medical Discipline of Suzhou SZXK202117

"National Tutorial System" Training Project for Young Health Backbone Talents in Suzhou QNGG2022030

Health Talents Training Project GSWS202099

More Information

Corresponding author: SHEN Qiang, E-mail: yanhuahuo110@163.com
Received Date: 2021-05-12
Rev Recd Date: 2021-08-17

Available Online: 2023-04-04

Publish Date: 2023-03-10

Abstract

Abstract

Objective This study aimed to conduct a preliminary molecular identification of pathogens in throat swab samples from a cluster of of cases with unexplained respiratory infections in Suzhou City. Methods Throat swab specimens of the outbreak cases were collected, and Real-time reverse transcription polymerase chain reaction (Real-time RT-PCR) method was employed to detect nucleic acid of common pathogens (influenza A/B viruses, parainfluenza viruses, respiratory syncytial viruses, coronaviruses, rhinoviruses, metapneumoviruses and adenoviruses) for clustered respiratory infections. Concurrently, FilmArray automatic medical PCR analysis system was used for multi-pathogen screening. High-throughput sequencing was performed on the throat swab specimens. And the sequenced results were verified by using specific kit to detect coxsackievirus A4 (CV-A4). Results Among the 18 throat swab samples submitted for testing during this outbreak, 4 were positive for CV-A4 by Real-time RT-PCR. CV-A4 specific gene fragments were identified in the throat swab samples via both Real-time RT-PCR and high-throughput sequencing method. Sequence analysis indicated that the complete genome sequence of the virus strain involved in this outbreak in Suzhou City shared the highest similarity (98.11%) to the MN964082 viral nucleic acid in the NCBI database. The VP1 protein amino acid sequence demonstrated a 98.03% similarity to the prototype strain AY421762. Six mutation sites were identified (T23V, A34T, S102A, A200T, I262V, and Y285H) when compared to AY421762. Conclusions CV-A4 has the potential to cause clustered respiratory infections. Enhanced health monitoring is necessary for vulnerable groups, particularly school-aged children, to facilitate prompt detection and management of such outbreaks.
- Real-time reverse transcription polymerase chain reaction,
- Mutation,
- Gene sequencing,
- Coxsackievirus A4

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References(15)

References

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