Lactoferrin ameliorates radiation-induced intestinal injury in mice by regulating ferroptosis

GUO Yaxin; XU Jiaying; WEI Wei; LI Deming; QIN Liqiang

doi:10.16462/j.cnki.zhjbkz.2023.04.016

Volume 27 Issue 4

Apr. 2023

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2023 > 27(4): 465-470

GUO Yaxin, XU Jiaying, WEI Wei, LI Deming, QIN Liqiang. Lactoferrin ameliorates radiation-induced intestinal injury in mice by regulating ferroptosis[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(4): 465-470. doi: 10.16462/j.cnki.zhjbkz.2023.04.016

Citation:

GUO Yaxin, XU Jiaying, WEI Wei, LI Deming, QIN Liqiang. Lactoferrin ameliorates radiation-induced intestinal injury in mice by regulating ferroptosis[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(4): 465-470. doi: 10.16462/j.cnki.zhjbkz.2023.04.016

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Lactoferrin ameliorates radiation-induced intestinal injury in mice by regulating ferroptosis

doi: 10.16462/j.cnki.zhjbkz.2023.04.016

1.
Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China
2.
State Key Laboratory of Radiation Medicine and Radiation Protection, Soochow University, Suzhou 215123, China

Funds:

The National Natural Science Foundation of China 82073482

The National Natural Science Foundation of China 82173502

More Information

Corresponding author: QIN Liqiang, E-mail: qinliqiang@suda.edu.cn
Received Date: 2022-09-08
Rev Recd Date: 2022-12-02

Available Online: 2023-04-28

Publish Date: 2023-04-10

Abstract

Abstract

Objective To explore the protective effect and mechanism of lactoferrin (Lf) on a radiation-induced intestinal injury. Methods Mice were randomly divided into the control group, 10 Gy group, and Lf+10 Gy group. On 7 d before irradiation, the Lf+10 Gy group was given 250 mg/kg Lf by gavage daily. Mice in the 10 Gy group and Lf+10 Gy group received 10 Gy abdominal irradiation. The mice were sacrificed 3.5 d after irradiation, and the samples were collected to determine the related indexes. Results Irradiation-induced intestinal injury. Lf intervention restored weight loss, hepatic oxidative stress, and pathological intestinal damage in mice. Lf significantly increased the number of Lgr5 and Ki67 positive cells, reflecting the proliferation and regeneration ability of small intestinal cells. Irradiation-induced ferroptosis in small intestinal tissue. Via the iron metabolism pathway, Lf intervention significantly downregulated the expression of intestinal divalent metal transporter (DMT1) and Fe²⁺ content; via the GSH/GPX4-lipid peroxidation pathway, Lf intervention significantly upregulated the intestinal solute carrier family 7 members (SLC7A11) and glutathione peroxidase 4 (GPX4) protein expression, downregulated acyl-CoA synthase long chain 4 (ACSL4) protein expression, and reduced malondialdehyde (MDA) levels. Conclusions Lf ameliorated radiation-induced intestinal injury, possibly due to the regulation of ferroptosis through iron metabolism and the GSH/GPX4-lipid peroxidation pathway.
- Lactoferrin,
- Radiation-induced intestinal injury,
- Ferroptosis

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References(18)

References

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