Construction of a multilocus map of single nucleotide polymorphisms in pneumoconiosis susceptibility genes

ZHU Fenglin; WANG Shuguang; JIN Yuting; CHEN Yiran; LIU Xinyu; YE Dongqing; WANG Jia

doi:10.16462/j.cnki.zhjbkz.2024.07.010

Volume 28 Issue 7

Jul. 2024

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ZHU Fenglin, WANG Shuguang, JIN Yuting, CHEN Yiran, LIU Xinyu, YE Dongqing, WANG Jia. Construction of a multilocus map of single nucleotide polymorphisms in pneumoconiosis susceptibility genes[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(7): 808-814. doi: 10.16462/j.cnki.zhjbkz.2024.07.010

Citation:

ZHU Fenglin, WANG Shuguang, JIN Yuting, CHEN Yiran, LIU Xinyu, YE Dongqing, WANG Jia. Construction of a multilocus map of single nucleotide polymorphisms in pneumoconiosis susceptibility genes[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(7): 808-814. doi: 10.16462/j.cnki.zhjbkz.2024.07.010

Citation:

ZHU Fenglin, WANG Shuguang, JIN Yuting, CHEN Yiran, LIU Xinyu, YE Dongqing, WANG Jia. Construction of a multilocus map of single nucleotide polymorphisms in pneumoconiosis susceptibility genes[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(7): 808-814. doi: 10.16462/j.cnki.zhjbkz.2024.07.010

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Construction of a multilocus map of single nucleotide polymorphisms in pneumoconiosis susceptibility genes

doi: 10.16462/j.cnki.zhjbkz.2024.07.010

ZHU Fenglin^{1, 2, 3},
WANG Shuguang⁴,
JIN Yuting⁴,
CHEN Yiran⁴,
LIU Xinyu⁴,
YE Dongqing^{2
,
,},
WANG Jia^{1, 2, 3
,
,}

1.
Joint National-Local Engineering Research Centre for Safe and Precise Coal Mining, Huainan 232001, China
2.
School of Public Health, Anhui University of Science and Technology, Hefei 231131, China
3.
Key Laboratory of Industrial Dust Prevention and Control & Occupational Health and Safety, Ministry of Education, Huainan 232001, China
4.
School of Medicine, Anhui University of Science and Technology, Huainan 232001, China

Funds:

University Natural Science Research Project of Anhui Province 2023AH051229

College Students Innovation and Entrepreneurship Training Program Project Anhui University of Science and Technology S202210361217

The Project was Supported by Independent Research Fund of Key Laboratory of Industrial Dust Prevention and Control & Occupational Health and Safety, Ministry of Education (Anhui University of Science and Technology) EK20211005

Scientific Research Start-up Foundation for High-level Talents of Anhui University of Science and Technology 13200005

Joint National-Local Engineering Research Centre for Safe and Precise Coal Mining EC2022009

More Information

Corresponding author: YE Dongqing, E-mail: ydqph@aust.edu.cn; WANG Jia, E-mail: wangjia@whu.edu.cn
Received Date: 2023-10-30
Rev Recd Date: 2024-04-28

Available Online: 2024-08-19

Publish Date: 2024-07-10

Abstract

Abstract

Objective To construct a single nucleotide polymorphisms (SNPs) multilocus map of pneumoconiosis susceptibility genes. Methods Based on RFLP technology, we integrated the reported SNPs of susceptibility genes, and designed different amplification fragments and digestion fragments which cover several different susceptibility gene locus. Then the multiple bands of different lengths obtained by agarose gel electrophoresis were used to identify the genetic typing rapidly. In addition, the cohort of coal workers′ pneumoconiosis(CWP) and dust receiving population in a mining area in northern Shaanxi was used for detection, and the genetic differentiation of different polymorphic loci of susceptible genes in the cohort was analyzed by fixation index (Fst). Results The SNPs of five susceptible genes were successfully constructed, which included IL-1β rs16944 T>C, IL-6 rs1800796 G>C, IL-8 rs4073 A>T, HSP70-1 rs562047 C>G, TGF-β rs1800469 T>C. These SNPs genotypes can be identified effectively. Fst analysis showed that the Fst of IL-1β rs16944 and TGF-β rs1800469 was > 0.05-0.15, indicating that, moderate genetic differentiation occurred. Conclusions The susceptibility loci rs16944 and rs1800469 were more susceptible to disease selection pressure, which was related to the susceptibility risk of pneumoconiosis.
- Pneumoconiosis,
- Susceptibility genes,
- Single nucleotide polymorphisms,
- Multilocus map

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References(20)

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