Epidemiological characterization and antimicrobial resistance of diarrhea Escherichia coli from acute diarrheal and healthy children in Guangzhou City, 2014-2015

Objective To investigate the epidemiological characteristics and antimicrobial resistance of diarrhea escherichia coli.Methods From June 2014 to June 2015, 143 and 83 stool samples were collected from children infected with acute diarrhea and healthy children respectively. Diarrhea escherichia coli was identified by biochemistry identification and PCR.Kirby Bauer methods(K-B disk diffusion methods) was adopted to evaluate antimicrobial resistance.Results The detection rates of enteropathogenic escherichia coli, enteroaggregative escherichia coli, enterotoxigenic escherichia coli and enteroinvasive escherichia coli in acute diarrhea children and health children specimens were 1.4% and 3.6%,2.8% and 2.4%,1.4% and 1.2%,0.7% and 1.2%,respectively. The age of 3-7 children with acute diarrhea had the highest detection rate of 26.7%, however,the age of 5-7 healthy children had the highest rate of 8.4%. The antimicrobial susceptibility tests showed that the resistance rates for tetracycline, ampicillin and cephalosporin were 52.6%-63.2%,and 5.3%-21.1%. In addition, the multi-drug resistance rate was 50.5%.Conclusions Diarrhea escherichia coli was detected in both acute diarrhea children and health children, and there were multiple infections,which hint diarrhea escherichia coli is an opportunistic infections bacteria. The result of antimicrobial resistance as well as multiple antimicrobial resistance are not optimistic. It is necessary to strengthen the surveillance of diarrheagenic escherichia coli and antimicrobial resistance, and providing a guide for clinical using antibiotic is also imperative.