The influence of the polymorphism of toll like receptor 7 rs179016 on the transcriptional activity

GAO Fan; ZHOU Yi-xin; WU Meng-ping; XIE Chao-nan; ZHANG Yun; ZHU Ping; YUE Ming

doi:10.16462/j.cnki.zhjbkz.2016.05.012

Volume 20 Issue 5

May 2016

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GAO Fan, ZHOU Yi-xin, WU Meng-ping, XIE Chao-nan, ZHANG Yun, ZHU Ping, YUE Ming. The influence of the polymorphism of toll like receptor 7 rs179016 on the transcriptional activity[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 477-480,485. doi: 10.16462/j.cnki.zhjbkz.2016.05.012

Citation:

GAO Fan, ZHOU Yi-xin, WU Meng-ping, XIE Chao-nan, ZHANG Yun, ZHU Ping, YUE Ming. The influence of the polymorphism of toll like receptor 7 rs179016 on the transcriptional activity[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 477-480,485. doi: 10.16462/j.cnki.zhjbkz.2016.05.012

Citation:

GAO Fan, ZHOU Yi-xin, WU Meng-ping, XIE Chao-nan, ZHANG Yun, ZHU Ping, YUE Ming. The influence of the polymorphism of toll like receptor 7 rs179016 on the transcriptional activity[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 477-480,485. doi: 10.16462/j.cnki.zhjbkz.2016.05.012

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The influence of the polymorphism of toll like receptor 7 rs179016 on the transcriptional activity

doi: 10.16462/j.cnki.zhjbkz.2016.05.012

1. Medical Department, the 202 Hospital, Shenyang Military Command, Shenyang 110003, China;
2. Epidemic Information Section, Center for Disease Control and Prevention of Shenyang Military Command, Shenyang 110034, China;
3. Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 210029, China;
4. Institute of Epidemiology and Microbiology, Military Medical Institute of Nanjing Military Command, Nanjing 210002, China;
5. Department of Medical Affairs, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;
6. Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

Received Date: 2015-12-26
Rev Recd Date: 2016-03-20

Abstract

Abstract

Objective Toll like receptor 7 (TLR7) plays an important role in sensing and provoking an immune response in hepatitis C virus (HCV) infection. This study aims to investigate the correlation of the polymorphism of TLR7 rs179016 with the transcription activity of TLR7. Methods 22 male volunteers were included in this study. The TLR7 mRNA expression was evaluated by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). The expression of the cultured HEK-293T cell lysates containing rs179016 C/G or empty plasmid was assessed using a dual-luciferase reporter gene system and compared. Results A lower TLR7 mRNA level was observed in subjects carrying G genotype than those carrying C genotype (t=-7.352,P<0.001). The fluorescence intensity of cells containing rs179016G was stronger than that of cells containing rs179016C. Conclusions Our data indicated that TLR7 rs179016 C genotype was associated with the lower transcription and translation of TLR7, which may contribute to an increased susceptibility to HCV infection.
- Toll-like receptor,
- Polymorphism,single nucleotide,
- Hepatitis C,chronic

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References(15)

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