Analysis on molecular epidemiology of Coxsackievirus A16 strains isolated from 2012 to 2014 in Jiangsu Province

YAO Xue-jun; SHAN Jun; JI Hong; LI Jing-xin; ZHANG Xue-feng; ZHU Feng-cai

doi:10.16462/j.cnki.zhjbkz.2016.05.013

Volume 20 Issue 5

May 2016

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YAO Xue-jun, SHAN Jun, JI Hong, LI Jing-xin, ZHANG Xue-feng, ZHU Feng-cai. Analysis on molecular epidemiology of Coxsackievirus A16 strains isolated from 2012 to 2014 in Jiangsu Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 481-485. doi: 10.16462/j.cnki.zhjbkz.2016.05.013

Citation:

YAO Xue-jun, SHAN Jun, JI Hong, LI Jing-xin, ZHANG Xue-feng, ZHU Feng-cai. Analysis on molecular epidemiology of Coxsackievirus A16 strains isolated from 2012 to 2014 in Jiangsu Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 481-485. doi: 10.16462/j.cnki.zhjbkz.2016.05.013

Citation:

YAO Xue-jun, SHAN Jun, JI Hong, LI Jing-xin, ZHANG Xue-feng, ZHU Feng-cai. Analysis on molecular epidemiology of Coxsackievirus A16 strains isolated from 2012 to 2014 in Jiangsu Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(5): 481-485. doi: 10.16462/j.cnki.zhjbkz.2016.05.013

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Analysis on molecular epidemiology of Coxsackievirus A16 strains isolated from 2012 to 2014 in Jiangsu Province

doi: 10.16462/j.cnki.zhjbkz.2016.05.013

1. Sub-center of Development Zones, Yancheng City Center for Disease Control and Prevention, Yancheng 224000, China;
2. Vaccine Clinical Evaluation Department, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China

Received Date: 2015-12-27
Rev Recd Date: 2016-02-21

Abstract

Abstract

Objective To analyze genetic characteristics of Coxsackievirus A16(CA16) strains isolated from Jiangsu Province from 2012 to 2014. Methods The clinical specimens were collected from children diagnosed as hand foot and mouth disease from 2012 to 2014, and CA16 virus strains were obtained by means of the specimens culture. The full-length VP1 gene fragments were amplified by reverse transcription polymerase chain reaction (RT-PCR) technique. Then, the amplified products were used for gene sequencing, sequence analysis were conducted by using bioinformatics software, phylogenetic tree was constructed and compared with CA16 reference strains for sequence homology. Results A total of 82 positive specimens were identified as CA16 from the confirmed HFMD patients and all CA16 strains' VP1 gene were sequenced successfully. For all the 82 strains, 9 strains were identified as B1a genotype and the others were B1b genotype, respectively. Sequence analysis results of these 82 strains showed that the nucleotide identity of 82 strains' VP1 genes ranged from 87.8% to 100.0% and the amino acid identity of 82 strains' VP1 proteins ranged from 97.5% to 100.0%. Compared with the ptototype strain CA16-G10,the homology of nucleotide sequences and amino acid sequences of VP1 gene were 75.2% to 78.2% and 90.5% to 91.9% respectively. Conclusions The CA16 strains isolated from 2012 to 2014 in jiangsu province belonged to subgenotype B1. The transmission mode showed that the dominant subgenotype of B1b and secondary subgenotype of B1a, were co-circulating together in Jiangsu Province. Both subgenotypes had close phylogenetic relationship.
- Hand foot and mouth disease,
- Coxsackievirus infections,
- Epidemiology, molecular

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References(24)

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