Genetic characteristics of hemagglutinin gene of H9N2 avian influenza viruses isolated from live poultry markets in Anhui Province from 2013 to 2018

HE Jun; YU Jun-ling; HE Lan; CHEN Qing-qing; HOU Sai; ZHU Meng; SU Bin

doi:10.16462/j.cnki.zhjbkz.2020.03.010

Volume 24 Issue 3

Jun. 2020

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2020 > 24(3): 297-302

HE Jun, YU Jun-ling, HE Lan, CHEN Qing-qing, HOU Sai, ZHU Meng, SU Bin. Genetic characteristics of hemagglutinin gene of H9N2 avian influenza viruses isolated from live poultry markets in Anhui Province from 2013 to 2018[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 297-302. doi: 10.16462/j.cnki.zhjbkz.2020.03.010

Citation:

HE Jun, YU Jun-ling, HE Lan, CHEN Qing-qing, HOU Sai, ZHU Meng, SU Bin. Genetic characteristics of hemagglutinin gene of H9N2 avian influenza viruses isolated from live poultry markets in Anhui Province from 2013 to 2018[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 297-302. doi: 10.16462/j.cnki.zhjbkz.2020.03.010

Citation:

HE Jun, YU Jun-ling, HE Lan, CHEN Qing-qing, HOU Sai, ZHU Meng, SU Bin. Genetic characteristics of hemagglutinin gene of H9N2 avian influenza viruses isolated from live poultry markets in Anhui Province from 2013 to 2018[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 297-302. doi: 10.16462/j.cnki.zhjbkz.2020.03.010

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Genetic characteristics of hemagglutinin gene of H9N2 avian influenza viruses isolated from live poultry markets in Anhui Province from 2013 to 2018

doi: 10.16462/j.cnki.zhjbkz.2020.03.010

1.
Department of Microbiological Laboratory,Anhui Provincial Center for Disease Control and Prevention,Key Laboratory for Medical and Health of the 13th Five-year Plan in Anhui Province,Hefei 230601,China
2.
Department of Acute Infectious Disease Prevention,Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,China

Funds: Health and Family Planning Commission Research Project in Anhui Province(2017jk005)

Received Date: 2019-07-26
Publish Date: 2020-06-10

Abstract

Abstract

Objective To analyze genetic characteristics of hemagglutinin(HA) gene of H9 N2 avian influenza viruses(AIVs) circulating in Anhui Province from 2013 to 2018. Methods All H9 N2 positive samples tested by real-time polymerase chain reaction(PCR) were inoculated into specific pathogen free(SPF) chicken eggs for isolation and purification. Viral RNA was reversely transcribed into cDNA and then amplified with gene specific primers. PCR products were sequenced and the gene sequences were analyzed using molecular and bioinformatics software. The DATAMONKEY online server was conducted to analyze selection pressure, and protein structure homology modelling was computed by the SWISS-MODEL server. Results 33 H9 N2 AIVs isolated from live poultry markets belonged to h9.4.2.5 in the phylogenetic tree. The receptor binding sites of HA gene at 183, 226 and 227 position were mutated into N, L and M, respectively. Meanwhile 189 and 190 sites presented with genetic polymorphism. Since 2015, all H9 N2 viruses in this study carried 6 potential N-linked glycosylation sites. It was found that position 160 of HA gene was subjected greater positive selection pressure, presented 7 spatial conformations at least. Conclusions The H9 N2 viruses isolated from live poultry markets in Anhui Province possess the molecular characteristics of infecting mammals and the ability of antigenic drift, so we need to pay more attention to the genetic characteristics of the viruses.
- H9N2,
- Avian influenza virus,
- Hemagglutinin,
- Genetic characteristics

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References(18)

References

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