Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer

ZHOU Mei-yu; YUAN Shuai; XIANG Ying; WU Long; WU Na; LI Cheng-ying; XU Bin; ZHANG Yao; CAI Tong-jian; MA Xiang-yu; YU Zu-bin; BAI Li; YANG Jing-yuan; LI Ya-fei

doi:10.16462/j.cnki.zhjbkz.2020.03.018

Volume 24 Issue 3

Jun. 2020

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2020 > 24(3): 341-347

ZHOU Mei-yu, YUAN Shuai, XIANG Ying, WU Long, WU Na, LI Cheng-ying, XU Bin, ZHANG Yao, CAI Tong-jian, MA Xiang-yu, YU Zu-bin, BAI Li, YANG Jing-yuan, LI Ya-fei. Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 341-347. doi: 10.16462/j.cnki.zhjbkz.2020.03.018

Citation:

ZHOU Mei-yu, YUAN Shuai, XIANG Ying, WU Long, WU Na, LI Cheng-ying, XU Bin, ZHANG Yao, CAI Tong-jian, MA Xiang-yu, YU Zu-bin, BAI Li, YANG Jing-yuan, LI Ya-fei. Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 341-347. doi: 10.16462/j.cnki.zhjbkz.2020.03.018

Citation:

ZHOU Mei-yu, YUAN Shuai, XIANG Ying, WU Long, WU Na, LI Cheng-ying, XU Bin, ZHANG Yao, CAI Tong-jian, MA Xiang-yu, YU Zu-bin, BAI Li, YANG Jing-yuan, LI Ya-fei. Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(3): 341-347. doi: 10.16462/j.cnki.zhjbkz.2020.03.018

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Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer

doi: 10.16462/j.cnki.zhjbkz.2020.03.018

1.
Department of Epidemiology, School of Public Health, Guizhou Medical University, Guiyang 550000, China
2.
Department of Epidemiology, College of Preventive Medicine, Army Medical University, Chongqing 400038, China
3.
Department of Thoracic Surgery, Xinqiao Hospital, Army Medical university, Chongqing 400037, China
4.
Department of Respiratory Disease, Xinqiao Hospital, Army Medical university, Chongqing 400037, China

Funds: The General Program of National Natural Science Foundation of China(81871896, 81672316); The Natural Science Foundation of Chongqing(CSTC2015jcyBX0110)

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Corresponding author: LI Ya-fei, E-mail:liyafei2008@hotmail.com
Received Date: 2019-09-05
Rev Recd Date: 2019-12-01
Publish Date: 2020-03-10

Abstract

Abstract

Objective To investigate the expression of long non-coding RNA SFTA1 P in non-small cell lung cancer(NSCLC)and its biological function in NSCLC cell lines. Methods Quantitative real time polymerase chain reaction(qRT-PCR) was used to detect the expression of SFTA1 P in 18 pairs of NSCLC tissues and adjacent normal tissues. The expression of SFTA1 P was detected by qRT-PCR in five different NSCLC cell lines(A549, SPCA1, H460, H1975 and H1299) and one normal lung epithelial cell line(HBE). The overexpression vector of SFTA1 P was designed and constructed. The overexpressed cell line was constructed by transfection, the effects of overexpression of SFTA1 P on proliferation, invasion and migration of NSCLC cells were detected by CCK-8 assay and transwell assay. Results The expression of SFTA1 P in NSCLC tissues was lower than that of adjacent normal tissues(t=2.158, P=0.043). SFTA1 P expression was detected in 5 strains of NSCLC cell lines and normal lung epithelial cell line. The expression of SFTA1 P was the lowest in A549 and H460 cell lines(t=5.769, P=0.004;t=5.772, P=0.004), and the highest in H1299 and H1975 cell lines(t=22.248, P < 0.001;t=11.814, P < 0.001). SFTA1 P overexpression cell models were successfully constructed using A549 and H460 cell lines(allP < 0.05). The overexpression of SFTA1 P could inhibit proliferation, invasion and migration of H460 and A549 cells((allP < 0.05). Conclusions SFTA1 P can affect the biological functions of NSCLC cells by inhibiting the proliferation, migration and invasion. SFTA1 P may play a role as a tumor suppressor gene in tumorigenesis and development.
- Non-small cell lung cancer,
- Long non-coding RNA,
- SFTA1P,
- Cell proliferation,
- Cell migration

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References(28)

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