Investigation on molecular characters of SARS-CoV-2 Nsp8 gene and protein

ZHANG Ling-han; JIN Yue-fei; CHEN Shuai-yin; YUE Li-min; DUAN Guang-cai

doi:10.16462/j.cnki.zhjbkz.2021.04.012

Volume 25 Issue 4

May 2021

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2021 > 25(4): 432-438

ZHANG Ling-han, JIN Yue-fei, CHEN Shuai-yin, YUE Li-min, DUAN Guang-cai. Investigation on molecular characters of SARS-CoV-2 Nsp8 gene and protein[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(4): 432-438. doi: 10.16462/j.cnki.zhjbkz.2021.04.012

Citation:

ZHANG Ling-han, JIN Yue-fei, CHEN Shuai-yin, YUE Li-min, DUAN Guang-cai. Investigation on molecular characters of SARS-CoV-2 Nsp8 gene and protein[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(4): 432-438. doi: 10.16462/j.cnki.zhjbkz.2021.04.012

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Investigation on molecular characters of SARS-CoV-2 Nsp8 gene and protein

doi: 10.16462/j.cnki.zhjbkz.2021.04.012

1.
Center for Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China
2.
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China

Funds:

Major National Science and Technology Projects 2018ZX10301407

More Information

Corresponding author: DUAN Guang-cai, E-mail: gcduan@zzu.edu.cn
Received Date: 2020-08-30
Rev Recd Date: 2020-11-08

Available Online: 2021-05-11

Publish Date: 2021-04-10

Abstract

Abstract

Objective To explore SARS-CoV-2 nsp8 genetic variation, Nsp8 protein structure, biological function and targeted drugs, and to lay foundation for establishing more effective prevention and control strategies. Methods Analyses of nsp8 genetic variability, physical and chemical characteristics, spatial structure, antigenic epitopes, biological function, and drug combined targets of Nsp8 were carried out using bioinformatics technology and large biological databases. Results Based on nsp8 sequences of 28 isolates of coronavirus of three species, evolutionary tree was successfully constructed. SARS-CoV-2 isolates showed 99%-100% conservation of nsp8 genes, less genetic distance to SARS than MERS isolates. Nsp8 had no signal peptide and transmembrane area. In reticulocytes in vitro, Nsp8 had a half-life of 4 h and was hydrophilic. A secondary model and a tertiary structure model were established. Linear B cell and CTL antigenic epitopes, phosphorylation and SUMB modification sites were found in Nsp8. Using the DrugBank database, four drugs targeted Nsp8 were obtained. Conclusions Nsp8 possesses the characteristics of typical antigens, participates in viral replication, and various isolates of the same species share high conservation of nsp8 gene, suggesting potential applications in researches on pathogenic mechanism, genotyping and prevention of this virus. Notably, this is the first report on Nsp8-targeted chemotherapeutic drugs, and the findings can be of considerable scientific significance and application value, under the conditions that measures with special effect for COVID-19 prevention and control are urgently needed.
- Severe acute respiratory syndrome coronavirus 2,
- Nsp8,
- Targeted drugs,
- Evolutionary tree,
- Antigenic epitope

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References(18)

References

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