Prevalence and risk factors of <i>optrA</i>-positive <i>Enterococcus</i> in healthy human gut

XIANG Qiu-mei; LYU Zi-quan; SHEN Ying-bo; WANG Yang; SHEN Jian-zhong; WU Si-ying; KE Yue-bin

doi:10.16462/j.cnki.zhjbkz.2022.11.012

Volume 26 Issue 11

Nov. 2022

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XIANG Qiu-mei, LYU Zi-quan, SHEN Ying-bo, WANG Yang, SHEN Jian-zhong, WU Si-ying, KE Yue-bin. Prevalence and risk factors of optrA-positive Enterococcus in healthy human gut[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(11): 1309-1314. doi: 10.16462/j.cnki.zhjbkz.2022.11.012

Citation:

XIANG Qiu-mei, LYU Zi-quan, SHEN Ying-bo, WANG Yang, SHEN Jian-zhong, WU Si-ying, KE Yue-bin. Prevalence and risk factors of optrA-positive Enterococcus in healthy human gut[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(11): 1309-1314. doi: 10.16462/j.cnki.zhjbkz.2022.11.012

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Prevalence and risk factors of optrA-positive Enterococcus in healthy human gut

doi: 10.16462/j.cnki.zhjbkz.2022.11.012

XIANG Qiu-mei^{1, 2},
LYU Zi-quan²,
SHEN Ying-bo^{3, 4},
WANG Yang⁴,
SHEN Jian-zhong⁴,
WU Si-ying^{1
,
,},
KE Yue-bin^{2
,
,}

1.
School of Public Health, Fujian Medical University, Fuzhou 350122, China
2.
Key Laboratory of Molecular Epidemiology, Shenzhen Center for Disease Control and Prevention, Shenzhen 510873, China
3.
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
4.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Funds:

National Key R&D Program of China 2018YFD0500303

Shenzhen Key Medical Discipline SZXK066

Research on the Produce and Transmission Mechanism of Foodborne Pathogens's Drug Resistance and the Influence of Human Health Risk JCYJ20170413101841798

More Information

Corresponding author: WU Si-ying, E-mail: fmuwsy@163.com; KE Yue-bin, E-mail: keyke@szu.edu.cn
Received Date: 2021-08-24
Rev Recd Date: 2022-01-26

Available Online: 2022-12-21

Publish Date: 2022-11-10

Abstract

Abstract

Objective To investigate the prevalence and risk factors of optrA-positive Enterococcus in the healthy people in Shenzhen City. Methods A total of 565 feces of the healthy pepople during 2018-2019 was cultured on enterococcal-selective plates to obtain florfenicol-resistant Enterococcus. MALDI Biotyper, Bruker, Germany (MALDI-TOF MS) was used for the species identification and PCR was used for optrA gene confirmation. People carrying optrA-positive enterococci were defined as positive cases and four times people with optrA-negative were randomly selected as control cases using the age of matching condition. A multivariate logistic regression model was used to analyze the risk factors of optrA-positive Enterococcus colonization in the human intestines. Results Overall, the prevalence of optrA-positive individuals was 18.10% (102/565, 95% CI: 14.90%-21.20%) in Shenzhen during 2018-2019. A total of 447 optrA-enterococci strains was isolated from the above samples, of which the Enterococcus faecalis was the most prevalent species (76.06%). Pork intake >50 g/d (OR=1.615, 95% CI: 1.017-2.565, P=0.042) and hospitalization within 3 months (OR=11.551, 95% CI: 2.153-61.963, P=0.004) were risk factors for optrA-positive Enterococcus colonization in the healthy human intestines. Conclusions optrA-positive Enterococcus have been already widespread in the human intestines in Shenzhen, and these bacteria may spread to humans through the food chain. Meanwhile, hospitalization will increase the risk of optrA-positive Enterococcus colonizing in the healthy hunman intestines.
- Linezolid,
- Enterococcus,
- optrA,
- Prevalence,
- Risk factors

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References(25)

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